Survey on Non-Human Primates & Mosquitoes Does not Provide Evidences of Spillover/Spillback between the Urban & Sylvatic Cycles of YFV & ZIKV Following Severe Outbreaks in Southeast Brazilby de Abreu et al.
The present study showed that there is no evidence that the ZIKV established an independent sylvatic cycle in the state of Rio de Janeiro, and provided new evidence that there was no urban transmission of Yellow Fever Virus in southeast Brazil during the current outbreak.
Findings in this study highlight the occurrence of the Fort Sherman virus (FSV) across a geographic range exceeding 7,000 km, surprising genomic conservation across a time span exceeding 50 years, M gene‐based reassortment events, and the existence of multiple animal hosts of FSV.
ZIKV cases confirmed by qRT-PCR indicate that the virus was circulating on Curaçao between at least December 2015 and March 2017, and on Bonaire between at least October 2016 and February 2017, with peak cases occurring in November 2016.
Comparison of Zika virus quantitative reverse transcription PCR results in single timepoint whole blood–plasma pairs from 227 patients with suspected Zika virus infection resulted in confirmation of 8 additional patients with Zika virus infection.
According to the authors, SuPReMe, therefore, represents an effective and promising option for the rapid generation of clonal recombinant populations of single-stranded positive-sense RNA viruses.